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ISSN : 1738-0294(Print)
ISSN : 2288-8853(Online)
Journal of Mushrooms Vol.11 No.1 pp.1-8

민자주방망이버섯(Lepista nuda )의 형태적 특성 및 교배에 관한 연구

정원순, 김종봉*
대구가톨릭대학교 대학원 생물학과

A study of morphological characteristics and hybridization on Lepista nuda

Jong Bong Kim*, Won soon Jung
Department of Biology, the Graduate School, Catholic University of Daegu


In this study, twelve of Lepista nuda were collected from various localities in Korea. Also thirteen exotic L. nudaspecies were collected from Japan, France, Switzerland and Portugal. Spores were isolated under optical microscope. Thesespores were placed on the surface of YM medium for inducing to germination. Eleven mating-groups were selected by morphologicalcharacters of fruit body such as size, color and stipe patterns. Intra-isolate crosses were made between two single-sporeisolates derived from mating-groups. Also, dikaryotic crossing using the isolates from L. nuda were carried out to evaluatedtetrakaryon formation. Cross-mating compatibility tests also verified its dikaryotic state by microscopic or molecular geneticobservation of clamp connection and Random Amplified Polymorphic DNA (RAPD) band pattern. To analyze the growth rateof hybrids and parents mycelium in dikaryons obtained from compatible mating groups were placed on PDA medium.Intra-isolate crosses determined eleven mating-groups within L. nuda. The typical clamp connection were mostly observed inmating-groups of Korean L. nuda in K1xK2, K1xK3, K1xK4, K1xK6, K1xK5, K2xK4, K2xK3, K2xK6, K3xK4, K4xK5 andK4xK6. Korean L. nuda type of dikaryon, shown to cross-incompatibility with L. sordida, it seemed that mating induce morerapidly than wild types in a view of growth rate. In conclusion, it would be useful to improve mass production with better morphologicalcharacteristics through a special mating of L. nuda.


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