큰느타리버섯의 저온적응성 형질에 관련된 SCAR Marker 개발
Development of a psychrophilic-SCAR marker for Pleurotus eryngii
(Received July 17, 2013 / Revised July 22, 2013 / Accepted September 16, 2013)
Abstract
Genomic DNAs of psychrophilic strains of Pleurotus eryngii were analyzed by randomly amplifiedpolymorphic DNA (RAPD) using OP-A, OP-B, OP-L, OP-P, OP-R and OP-S3 primers to develop the strain-specificDNA marker. A unique DNA fragment with the size of 480 bp was yielded by OP-S3 primer from the psychrophilicstrain. A sequence characterized amplified region (SCAR) marker, designated as OP-S3-1, was designed on the basisof the determined sequence. The PCR analysis with the OP-S3-1 primer showed that this SCAR marker can clearlydistinguish the psychrophilic strains from the control strains.
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2.류재산, 김민근, 권진혁, 조숙현, 김낙구, 노치웅, 이춘희, 노현수, 이현숙. 2007. 큰느타리버섯의 자실체 생육특성. 한국균학회지. 35 : 47-53.
3.서경인, 장갑열, 유영복, 박순영, 김광호, 공원식. 2011. 느타리 버섯에서 수한 품종 특이 SCAR marker 개발. 한국균학회지. 39 : 31-38.
4.Bang, K. H., Sung, J. S., Park, C. H., Jin, D. C., Park, C. G., Yu, H. S., Park, H. W. and Seong, N. S. 2004. Discrimination of Atractylodes rhizome white using anatomical characteristics and SCAR Markers. Kor. J. Medicinal Crop Science 7 : 53-59.
5.Hashimoto, K. and Takahashi, Z. 1974. Studies on the growth of Pleurotus ostreatus. Mushroom Science 9 : 585-593.
6.Hongyan, S., Lei, W., Yihe, G., Ermei, F., Jie, S. and Linde, L. 2008. Development of strain-specific SCAR markers for authentication of Ganoderma lucidum. World J. Microbiol. Biotechnol. 24 : 1223-1226.
7.Koveza, O. V. and Gostimsky, S. A. 2005. Development and study of SCAR marker in pea (Pisum sativum L.). Russian J. of Genetics 41 : 1254-1261.
8.Lee, M. Y., Doh, E. J., Park, C. H., Kim, Y. H., Kim, E. S., Ko, B. S. and Oh, S. E. 2006. Development of SCAR marker discrimination of Artemisia princeps and Artemisia argyi from other Artemisia Herbs. Biol. Pharm. Bull. 29 : 629-633.
9.Lewinsohn, D., Nevo, E., Hadar, Y., Wasser, S. P. and Beharav, A. 2000. Ecogeographical variation in the Pleurotus eryngii complex in Israel. Mycol. Res. 104 : 1184-1190.
10.Paran, I. and Michelmore, R. W. 1991. Identification of markers linked to disease resistance genes by bulked segregant analysis: a rapid method to detect markers in specific genomic regions by using segregating populations. Proc. Natl. Acad. Sci. USA 88: 9828-9832.
11.Paran, I. and Michelmore, R. W. 1993. Development of reliable PCR-based, markers linked to downey mildew resistance genes in lettuce. Theor. Appl. Genet. 85 : 985-993.
12.Qin, L. H., Tan, Q. I., Chen, M. J. and Pan, Y. J. 2006. Use of intersimple sequence repeats markers to develop strainspecific SCAR markers for Lentinula edodes. FEMS Microbiol. Lett. 257 : 112-116.
13.Rajarathnam, R. and Bano, Z. 1987. Pleurotus mushrooms. Part 1A. Morphology, Lifecycle, Tanxonomy. Breeding and cultivation. CRC Critical in Food Science and Nutrition. 26 : 157-222.
14.Song, Y. J., Jeong, M. J., Kim, B. G., Rho, Y. D., Ryu, J. C. and Yoo, Y. B. 1996. Genetic variability of Pleurotus ostreatus monospore isolates by random amplified polymorphic DNA analysis. Kor. J. Mycol. 24 : 186-205.