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ISSN : 1738-0294(Print)
ISSN : 2288-8853(Online)
Journal of Mushrooms Vol.11 No.3 pp.124-130

Cultural characteristics of Cordyceps militaris strain ‘Yedang 3’ on various media and nutritional conditions

Byung-Joo Lee1*, Mi-Ae Lee1, Yong-Gyun Kim1, Kwang-Won Lee1, Young-Sang Choi2, Byung-Eui Lee3, Ho-Yeon Song4
1Crop Research Division, Chungcheongnam-do Agricultural Research & Extension Services, Yesan 340-861, Korea
2Yedang Mushroom Institute, Yesan 305-764, Korea
3Industry Academy Cooperation Foundation, Soonchunghyang University, Asan 336-745, Korea
4College of Medicine, Soonchunghyang University, Chunan 330-930, Korea
(Received September 13, 2013 / Revised September 21, 2013 / Accepted September 27, 2013)


The effects of environmental and nutritional requirement such as temperature, pH, different media,carbon, nitrogen, and carbon-to-nitrogen ratio on the mycelial growth of Cordyceps militaris strain ‘Yedang 3’ werestudied. The optimum temperature and pH for the growth of mycelium were 20-25℃ and pH 6-7, respectively.Out of ten media tested, mushroom complete media (MCM) was the best medium for fast mycelial growth, andSabouraud’s dextrose agar yeast extract (SDAY), malt extract yeast extract agar (YMA) also were favored. Thecolor and shape of colonies varied in different media. The best carbon sources for mycelial growth were fructose,mannitol, and sucrose, whereas the best nitrogen sources were tryptone and peptone. However, mycelia grew slowlyin inorganic nitrogen compounds such as NH4Cl, (NH4)2SO4, NH4NO3, and NaNO3. The optimum C:N ratioobserved on the culture media was 30-40 range. These results provided basic information on cultural characteristicsof vegetative growth and might be useful for spawn production in Cordyceps militaris.


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