Lentinula edodes is known by oak mushroom. It has been favored as delicious and nutritious food and the lowcaloriefood with a high nutritional value. It is also functional food since it contains a material well-known for its medicinalbenefits. Since the growth and quality of oak mushrooms are sensitively affected by environmental conditions, an adequateenvironmental control is very essential to improve the yield and quality under protected cultivation. The main objectives of thestudy were to investigate cultural characteristics of mycelial growth and in vitro fruiting of Lentinula edodes. The optimum culturemedia for mycelial growth of L. edodes were PDA and MYA. Similarly, optimum temperature was 25oC. Malt extract(2%) andyeast extract(0.2%) were optimum carbon and nitrogen sources. Optimal culture period was 110~120 days in sawdust medium.Mycelial growth in medium(61 mm/7 days) Quercus mongolica extract the most good. Among different five log types, highestmycelial growth and fruiting productivity were observed in Quercus variabilis sawdust(20.9%).