Gloeostereum incarnatum is an edible and medicinal mushroom belongs to Family Cyphellaceae of Polyporales,Basidiomycota. The purpose of this study was to investigate the free radical scavenging, anti-inflammatory, and melanin synthesisinhibitory activities of fruiting bodies of Gloeostereum incarnatum. In the free radical scavenging activities, the mushroom extractsshowed good 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging and chelating activity on the ferrous ions comparedwith the positive control, BHT. The mushroom extract suppressed nitric oxide (NO) production in LPS-induced RAW 264.7macrophage cells in dose dependant manners. Significant reduction of paw edema of rats were observed at 2~6 h afteradministration with 50 mg/kg of the methanol and hot-water extracts, which were comparable with treatment of 5 mg/kg ofindomethacin, the positive control. The melanin synthesis of Melanoma B16/F10 cells treated with 100 μg/mL of the methanoland hot water extracts decreased melanin concentration to 50% and 45% compared with the control, arbutin. Therefore, theexperimental results showed that methanol and hot-water extracts of Gloeostereum incarnatum fruiting bodies might be used forgood sources of anti-inflammatory, free radical scavenging, and skin whitening agents for human health.