ISSN : 1738-0294(Print)
ISSN : 2288-8853(Online)
ISSN : 2288-8853(Online)
Journal of Mushrooms Vol.12 No.3 pp.181-186
DOI : https://doi.org/10.14480/JM.2014.12.3.181
DOI : https://doi.org/10.14480/JM.2014.12.3.181
느티만가닥버섯의 재래종과 감마선 돌연변이체들의 유전적 변이
Genetic variation of local varieties and mutants groups induced by gamma ray in Hypsizigus marmoreus
Received July 17, 2014, Revised August 12, 2014, Accepted August 12, 2014
Abstract
This research was carried out to analyze the genetic variation of 18 wild strain, 2 breed varieties and 20 mutants ofHypsizygus marmoreus by random amplification of polymorphic DNA(RAPD). Also, 3 strains of Lyophyllum decartes and 1 strainof Lyophyllum shimeji were used. These mushrooms were collected from korea, china, Taiwan and Japan. Spores of H.marmoreus JV-2 strain were irradiated by gamma ray for mutagenesis. 40 kind of primers were used for this reaserch. Number ofreaction primer were 31. Electrophorectic patterns of RAPD showed genetic variation. In phylogenetic tree, they were dividedinto seven group. Discriminative differences were observed between wild strain and mutants in H. marmoreus. These resultsmight suggest that these primers and gamma ray irradiation of spores were useful tools for developing new strain for mushroom.