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ISSN : 1738-0294(Print)
ISSN : 2288-8853(Online)
Journal of Mushrooms Vol.12 No.4 pp.357-361
DOI : https://doi.org/10.14480/JM.2014.12.4.357

표고버섯좀나방에 대한 곤충병원성 선충의 병원성 검정

김형환, 김동환, 조명래, 야창열, 강택준, 전성욱, 송진선, 정영학, 박해웅, 박정규, 추호렬
국립원예특작과학원 원예특작환경과
경상대학교 응용생명과학부, 농업생명과학연구원
세계김치연구소

Pathogenicity bioassay of entomopathogenic nematode, Steinernema carpocapsae GSN1 strain (Nematoda: Steinernematidae) on the shiitake fungus moth, Morophagoides moriutii (Lepidoptera: Tineidae)

Hyeong Hwan Kim, Dong Hwan Kim, Myung Rae Cho, Chang Yeol Yang, Taek Jun Kang, Sung Wook Jeon, Jin Sun Song, Young Hak Jung, Hae Woong Park, Chung Gyoo Park, Ho Yul Choo
Horticultural & Herbal Crop Environment Division, National Institute of Horticultural & Herbal Science, RDA, Suwon 441-440, Republic of Korea
Division of Applied Life Science (BK21+)/Institute of Agriculture and Life Sciences,
Gyeongsang National University, Jinju, Gyeongnam 660-701, Republic of Korea
World Institute of Kimchi, An Annex of Korea Food Research Institute, Gwangju 503-360, Republic of Korea
Received October 30, 2014 Revised December 26, 2014 Accepted December 31, 2014

Abstract

Pathogenicity of Steinernema carpocapsae GSN1 strain was evaluated against Morophagoides moriutii larvae. In Petridishtests, insect mortality by the nematode was dose dependent, which increased with dose from 5 to 160 infectivejuveniles(IJs)/larva. Pathogenicity against fourth-instar larvae was higher than the rate of corresponding second- and third-instarlarvae, showing 100% insect mortality with the dose of 40 IJs/4th instar larva and 80 IJs/2nd or 3rd instar larvae. Lethal concentrationvalues at 50% (LC50) of S. carpocapsae GSN1 strain were 4.2 IJs/2nd instar larva; 8.5 IJs/3rd instar larva; and 2.3 IJs/4th instar larva,respectively. The number of nematodes established in M. moriutii larvae after infection increased in the increment of dose andinsect developmental stage. The highest number of nematodes was harvested from fourth instar larvae of M. moriutii at a dose of160 IJs per larva, showing 22.5 nematodes per insect larva. Nematode reproductive capacity was related to insect developmentalstage, showing 6,335 IJs/2nd instar larva, 21,660 IJs/3rd instar larvae, and 88,700 IJs/4th instar larvae.

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