Journal Search Engine
Search Advanced Search Adode Reader(link)
Download PDF Export Citaion korean bibliography PMC previewer
ISSN : 1738-0294(Print)
ISSN : 2288-8853(Online)
Journal of Mushrooms Vol.13 No.1 pp.79-83
DOI : https://doi.org/10.14480/JM.2015.13.1.79

베타글루칸 함량이 높은 큰느타리버섯 선발을 위한 SCAR marker 개발

김수철, 김혜수, 조용운, 류재산1, 조수정*
경남과학기술대학교 제약공학과, 1경상남도농업기술원 친환경연구과

Development of strain-specific SCAR marker for selection of Pleurotus eryngii strains with higher -glucan

Soo Jeong Cho*, Su Cheol Kim, Hye Soo Kim, Yong Un Cho, Jae-San Ryu1
Department of Pharmaceutical Engineering, Gyeongnam National University of Science and Technology, 33 Dongjin-ro, Jinju 660-758, Korea
1Eco-friendliness Research Department, Gyeongnam-do Agricultural Rearch and Extension Services, Jinju 660-360, Korea

Abstract

In this study, SCAR marker that differentiates Pleurotus eryngii strains with higher β-glucan from control strain was developed. Genomic DNAs of 9 control strains of Pleurotus eryngii and 9 Pleurotus eryngii strains with higher β-glucan were analyzed by bulked segregant analysis (BSA) using randomly amplified polymorphic DNA (RAPD). One-hundred twenty RAPD primers were screened on bulked DNA samples and a unique DNA fragment with the size of 91 bp was yielded by OP-R03 primer from the Pleurotus eryngii strains with higher β-glucan. A sequence characterized amplified region (SCAR) marker, designated as OP-R03-1-F and OP-R03-1-R, was designed on the basis of the determined sequence. The PCR analysis with the OP-R03-1 primer showed that this SCAR marker can clearly distinguish the Pleurotus eryngii strains with higher β-glucan from the control strains.

Reference