We found about cultivation light environment conditions of Neolentinus lepideus. For the high quality of N. lepideus,lights-out was useful. In the lights-out, the diameter of pileus of N. lepideus was smaller, and the length and the thickness ofstipes were longer and larger than in that of lighting. At the LED effect, the orange and red LEDs prolonged the cultivation periodin N. lepideus. The yields of N. lepideus under the white, blue and green LEDs were higher, and the number of available stipesof N. lepideus under the white and blue LEDs was higher than that of other LEDs. The diameter of pileus of N. lepideus underthe white, blue and green LEDs was higher, and the length and the thickness of stipes under the orange and red LEDs was higherthan those of other LEDs.