ISSN : 1738-0294(Print)
ISSN : 2288-8853(Online)
ISSN : 2288-8853(Online)
Journal of Mushrooms Vol.13 No.2 pp.129-134
DOI : https://doi.org/10.14480/JM.2015.13.2.129
DOI : https://doi.org/10.14480/JM.2015.13.2.129
Characteristics and breeding of a new oyster mushroom (Pleurotus ostreatus) variety ‘Chunhwashim’ by using cytoplasmic hybrid
June 19, 2015
June 24, 2015
June 29, 2015.
Abstract
Hybrid strains was selected by crossing between dikaryotic strain ASI 0628 (Dagul) bred from ASI 2596 (Suhan No.3)and ASI 2782(Black pileus mutant) and monokaryotic strain ASI 2344-84. The strain 84 that shown the best culturalcharacteristics was selected to be a new cytoplasmic hybrid variety and named as ‘Chunhwashim’. The ‘Chunhwashim’ shownincompatibility among them forming the confrontation growth line against parental strains ASI 0628 (Dagul) and ASI 2344(chunchu 2ho). Fruiting body produced about 161.4±4.8 g per bottle. And also the individual generation of fruit body is multiplethan chunchu2ho as 40.9. The ‘Chunhwashim’ was cytoplasmic hybrid included hybrid DNA bands of parental strains ASI 0628(Dagul) and ASI 2344 (chunchu 2ho) by Random Amplified Polymorphic DNA (RAPD) primer, and mitochondria DNA band ofmonokaryotic ASI 2344 (chunchu 2ho) using mitochondria microsatellite DNA marker. This new cytoplasmic hybrid variety'Chunhwashim' of oyster mushroom is characterized by multiple of individual generation and deeply grey color of pileus.