The brix% of ASI 52822 resulted in the highest solid solubility. ASI 53399, ASI 50167, and ASI 52822 displayed high carbohydrate contents, whereas GBGA 01, GBGA 02, and ASI 52823 exhibited the half those values. The highest protein content was measured in ASI 52822. The antioxidant activity in the case of the hydrothermal method was lower than that for ascorbic acid, although it revealed more than 65% antioxidant activity. Furthermore, ASI 53399 and ASI 52822 exhibited antioxidant activities of 83.8% and 81.3%, respectively. To confirm the anticancer activity of the Ganoderma applanatum extract, we measured the original extract and also prepared a 10-time extract. The 10-time extract yielded strong anticancer activity, although the original extract did not exhibit any.